Thermoprotection by glycine betaine and choline
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چکیده
منابع مشابه
Thermoprotection by glycine betaine and choline.
Glycine betaine is mostly known as an osmoprotectant. It is involved in the osmotic adaptation of eukaryotic and bacterial cells, and accumulates up to 1 M inside cells subjected to an osmotic upshock. Since, like other osmolytes, it can act as a protein stabilizer, its thermoprotectant properties were investigated. In vitro, like protein chaperones such as DnaK, glycine betaine and choline pro...
متن کاملGenetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor.
Synthesis of the compatible solute glycine betaine confers a considerable degree of osmotic stress tolerance to Bacillus subtilis. This osmoprotectant is produced through the uptake of the precursor choline via the osmotically inducible OpuB and OpuC ABC transporters and a subsequent two-step oxidation process by the GbsB and GbsA enzymes. We characterized a regulatory protein, GbsR, controllin...
متن کاملMetabolism of trimethylamine, choline, and glycine betaine by sulfate-reducing and methanogenic bacteria in marine sediments.
The response of methanogenesis and sulfate reduction to trimethylamine, choline, and glycine betaine was examined in surface sediments from the intertidal region of Lowes Cove, Maine. Addition of these substrates markedly stimulated methanogenesis in the presence of active sulfate reduction, whereas addition of other substrates, including glucose, acetate, and glycine, had no effect on methane ...
متن کاملThermoprotection of Bacillus subtilis by exogenously provided glycine betaine and structurally related compatible solutes: involvement of Opu transporters.
Bacillus subtilis possesses five osmotically regulated transporters (Opu) for the uptake of various compatible solutes for osmoprotective purposes. We have now found that compatible solutes also function as thermoprotectants for B. subtilis. Low concentrations of glycine betaine enhanced the growth of the B. subtilis wild-type strain JH642 at its maximal growth temperature (52 degrees C) but di...
متن کاملHomeostasis and catabolism of choline and glycine betaine: lessons from Pseudomonas aeruginosa.
Most sequenced bacteria possess mechanisms to import choline and glycine betaine (GB) into the cytoplasm. The primary role of choline in bacteria appears to be as the precursor to GB, and GB is thought to primarily act as a potent osmoprotectant. Choline and GB may play accessory roles in shaping microbial communities, based on their limited availability and ability to enhance survival under st...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1999
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-145-9-2543